Controlling Bdellovibrio bacteriovorus Gene Expression and Predation Using Synthetic Riboswitches
نویسندگان
چکیده
منابع مشابه
Role of Type IV Pili in Predation by Bdellovibrio bacteriovorus
Bdellovibrio bacteriovorus, as an obligate predator of Gram-negative bacteria, requires contact with the surface of a prey cell in order to initiate the life cycle. After attachment, the predator penetrates the prey cell outer membrane and enters the periplasmic space. Attack phase cells of B. bacteriovorus have polar Type IV pili that are required for predation. In other bacteria, these pili h...
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Early electron microscopy and more recent studies in our laboratory of Bdellovibrio bacteriovorus cells indicated the presence of narrow fibers at the nonflagellar pole of this unusual predatory bacterium. Analysis of the B. bacteriovorus HD100 genome showed a complete set of genes potentially encoding type IV pili and an incomplete gene set for Flp pili; therefore, the role of type IV pili in ...
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The availability of the complete genome sequence of Bdellovibrio bacteriovorus provides an opportunity for investigating genes that play a significant role in predation. Using two independently derived facultatively predatory Bdellovibrio strains, we have designed a method to cultivate and screen transposon insertion mutants in 96-well microtiter dishes. Transposon insertion mutants were produc...
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Biofilms are matrix-enclosed microbial communities that grow at interfaces. They are highly robust and exhibit significant phenotypic changes that render them resistant to many antibacterial agents that can kill their freeswimming counterparts. Researchers have tried to find an effective, alternative and bio-friendly way to eliminate biofilms. Previous investigations in this group demonstrated ...
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The recently sequenced genome of the predatory delta-proteobacterium Bdellovibrio bacteriovorus provides many insights into its metabolism and evolution. Because its genes are reasonably uniform in G+C content, it was suggested that B. bacteriovorus actively resists recombination with foreign DNA and horizontal transfer of DNA from other bacteria. To investigate this further, we carried out a v...
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ژورنال
عنوان ژورنال: ACS Synthetic Biology
سال: 2017
ISSN: 2161-5063,2161-5063
DOI: 10.1021/acssynbio.7b00171